Description:
Sodium cyclohexylsulfamate (CHS-Na), also known as sodium cyclamate, was a widely used sweetening agent. It was banned for general use in 1969 because of the suspicion of carcinogenicity and metabolic conversion to cyclohexylamine (CHA), a toxic substance. A Pseudomonas species can desulfate CHS-Na to CHA using the enzyme cyclamate sulfamatase ([http://www.ncbi.nlm.nih.gov/pubmed/4209783|Nimura et al., 1974]). Also, this bacterium can deamine cyclohexylamine (CHA) to cyclohexanone (CHnone) using the enzyme cyclohexylamine oxidase (CHA-oxidase) ([http://www.ncbi.nlm.nih.gov/pubmed/18451|Tokieda et al., 1977]).